Protein Database
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The lecture covers various protein databases and bioinformatics resources, including UniProt, PDB, SCOP, CATH, and others, emphasizing their roles in protein sequence and structure analysis. It highlights tools for sequence alignment, motif discovery, and protein classification, providing essential definitions and functionalities of each database. Additionally, the document outlines the architecture and useful applications of these resources in computational biology.
![PROTEIN TERMS & DEFINITIONS
• Protein Sequence – 20 a.a. characters [A, C, D, E, F, G, H, I, K, L, M, N, P, Q, R, S, T, V, W, Y] in
sequence
• Protein Structure – 3D of atomic co-ordinates [x-axis, y-axis, z-axis]
• Types of Biological Databases – [Raw Database = Plain text, Object-oriented Database = Table
(Records), Relational Database = Table of tables]
• 3D Atom Model – [Sphere = Atom, Cylinder = Bond, Dotted Line = Bond Interaction]
• Sequence Alignment – [Match = Similar Character, Mismatch = Dissimilar Character, Gap = No
Substitute Character, Word = Sub-string, Sequence = Super-string, Score = Rating, Identity =
Similar in function]
• Motif – Short, conserved sequence associated with a distinct function.
• Domain – Evolutionarily conserved sequence region that corresponds to a structurally
independent 3D unit associated with a particular functional role. It is usually much larger than a
motif.
• Pattern – Sequence with symbol representation for a expression. Example: N{P}[ST]{P}
• Regular Expression – Representation format for a sequence motif, which includes positional
information for conserved and partly conserved residues. Similar to Pattern, but applies to MSA.
• Profile – Scoring matrix that represents a multiple sequence alignment. It contains probability or](https://image.slidesharecdn.com/proteindatabase-160419051849/85/Protein-Database-3-320.jpg)
![SEQUENCE RETRIEVED FROM NBRF/PIR IN FASTA FILE
FORMAT
>F7VJQ1 APRIO_HUMAN Alternative prion protein [Homo
sapiens]
MEHWGQPIPGAGQPWRQPLPTSGRWWLGAASWWWLGAASWWWLGAAPWWWLGTASWWWL
G
SRRWHPQSVEQAE](https://image.slidesharecdn.com/proteindatabase-160419051849/85/Protein-Database-15-320.jpg)
![MOTIF
• Motif is a search service provided by GenomeNet to search with a protein query
sequence against Motif Libraries.
• Supports several motif databases such as Prosite, BLOCKS, ProDom, Pfam, and
PRINTS.
• Allows you to search protein sequence libraries with your patterns.
• Each residue must be separated with - (minus sign).
• x represents any amino acids.
• [DE] means either D or E.
• {FWY} means any amino acids except for F, W and Y
• A(2,3) means that A appears 2 to 3 times consecutively.
• The pattern string must be terminated with . (period).
For example, C-x-{C}-[DN]-x(2)-C-x(5)-C-C.
• Generates a profile from a set of multiple aligned sequences using PFMake or
HMMBuild, respectively.http://www.genome.jp/tools/motif/](https://image.slidesharecdn.com/proteindatabase-160419051849/85/Protein-Database-28-320.jpg)
Protein Database
- 1. LECTURE TOPIC: PROTEIN DATABASE T. ASHOK KUMART. ASHOK KUMAR HEAD, DEPARTMENT OF BIOINFORMATICSHEAD, DEPARTMENT OF BIOINFORMATICS NOORUL ISLAM COLLEGE OF ARTS ANDNOORUL ISLAM COLLEGE OF ARTS AND SCIENCESCIENCE KUMARACOIL, THUCKALAY - 629180KUMARACOIL, THUCKALAY - 629180
- 2. TOPICS COVERED • Protein Terms & Definitions – Computational biology aspect of protein • ExPASy – SIB Bioinformatics Resource Portal (http://www.expasy.org) • UniProt/Swiss-Prot – A comprehensive, non-redundant, expert manually annotated protein sequence database (http://www.uniprot.org/) • NBRF/PIR– A comprehensive, non-redundant, expertly manually annotated, fully classified and extensively cross-referenced protein sequence database (http://pir.georgetown.edu/) • PDB– A single worldwide repository of information about the 3D structures of large biological molecules, including proteins and nucleic acids (http://rcsb.org/pdb) • SCOP– Knowledge-based expert analysis and classification of proteins that are structurally characterized and deposited in the Protein Data Bank (http://scop2.mrc-lmb.cam.ac.uk/) • CATH– A hierarchical domain classification of protein structures in the Protein Data Bank (http://www.cathdb.info/) • MOTIF – Finds sequence motifs in a query sequence, also provides functional and genomic information of the found motifs using DBGET and LinkDB as the hyperlinked annotations (http://www.genome.jp/tools/motif/) • Pfam – Database of protein HMM profiles that define domain families (http://pfam.xfam.org/) • PROSITE – Database of protein motifs expressed as patterns or profiles
- 3. PROTEIN TERMS & DEFINITIONS • Protein Sequence – 20 a.a. characters [A, C, D, E, F, G, H, I, K, L, M, N, P, Q, R, S, T, V, W, Y] in sequence • Protein Structure – 3D of atomic co-ordinates [x-axis, y-axis, z-axis] • Types of Biological Databases – [Raw Database = Plain text, Object-oriented Database = Table (Records), Relational Database = Table of tables] • 3D Atom Model – [Sphere = Atom, Cylinder = Bond, Dotted Line = Bond Interaction] • Sequence Alignment – [Match = Similar Character, Mismatch = Dissimilar Character, Gap = No Substitute Character, Word = Sub-string, Sequence = Super-string, Score = Rating, Identity = Similar in function] • Motif – Short, conserved sequence associated with a distinct function. • Domain – Evolutionarily conserved sequence region that corresponds to a structurally independent 3D unit associated with a particular functional role. It is usually much larger than a motif. • Pattern – Sequence with symbol representation for a expression. Example: N{P}[ST]{P} • Regular Expression – Representation format for a sequence motif, which includes positional information for conserved and partly conserved residues. Similar to Pattern, but applies to MSA. • Profile – Scoring matrix that represents a multiple sequence alignment. It contains probability or
- 4. EXPASY • ExPASy (Expert Protein Analysis System) is a bioinformatics resource portal operated by the Swiss Institute of Bioinformatics (SIB). • ExPASy was the first website of the life sciences. • Extensible and integrative portal for accessing many scientific resources, databases and software tools. • Wide range of resources in many different domains, such as proteomics, genomics, phylogeny/evolution, systems biology, population genetics, transcriptomics, etc. • Proteomics server to analyze protein sequences and structures and 2D Page gel electrophoresis. • Databases, online and offline software tools are hosted by different groups of the SIB and partner institutions. --- CFSSP • ExPASy references the protein sequence knowledgebase, UniProtKB/Swiss-Prot, and its computer annotated supplement, UniProtKB/Trembl.
- 5. ARCHITECTURE OF UNIPROT/SWISS-PROT • Universal Protein Resource (UniProt) is a comprehensive resource for protein sequence and annotation data • The UniProt databases are the UniProt Knowledgebase (UniProtKB), the UniProt Reference Clusters (UniRef), and the UniProt Archive (UniParc) • UniProt Metagenomic and Environmental Sequences (UniMES) database is a repository specifically developed for metagenomic and environmental data
- 6. BACKGROUND OF UNIPROT/SWISS-PROT • UniProt is a collaboration between the European Bioinformatics Institute (EMBL-EBI), the Swiss Institute of Bioinformatics (SIB) and the Protein Information Resource (PIR) • EMBL-EBI and SIB together used to produce Swiss-Prot and TrEMBL, while PIR produced the Protein Sequence Database (PIR-PSD) • Translated EMBL Nucleotide Sequence Data Library (TrEMBL) was originally created because sequence data was being generated at a pace that exceeded Swiss-Prot's ability to keep up • PIR maintained the PIR-PSD and related databases, including iProClass, a database of protein sequences and curated families http://www.uniprot.org/
- 10. UNIPROT/SWISS-PROT FILE FORMAT Line code Content Occurrence in an entry ID Identification Once; starts the entry AC Accession number(s) Once or more DT Date Three times DE Description Once or more GN Gene name(s) Optional OS Organism species Once or more OG Organelle Optional OC Organism classification Once or more OX Taxonomy cross-reference Once OH Organism host Optional RN Reference number Once or more RP Reference position Once or more RC Reference comment(s) Optional RX Reference cross-reference(s) Optional RG Reference group Once or more (Optional if RA line) RA Reference authors Once or more (Optional if RG line) RT Reference title Optional RL Reference location Once or more CC Comments or notes Optional DR Database cross-references Optional PE Protein existence Once KW Keywords Optional FT Feature table data Once or more in Swiss-Prot, optional in TrEMBL SQ Sequence header Once (blanks) Sequence data Once or more
- 11. NBRF/PIR • The Protein Information Resource (PIR) was established in 1984 by the National Biomedical Research Foundation (NBRF) as a resource to assist researchers in the identification and interpretation of protein sequence information. • In 2002 PIR, along with its international partners, EBI and SIB, were awarded a grant from NIH to create UniProt, by unifying the PIR-PSD, Swiss-Prot, and TrEMBL databases. • As of 2010, PIR offers a wide variety of resources mainly oriented to assist the propagation and standardization of protein annotation: PRO, iProClass, iProLINK. http://pir.georgetown.edu/
- 12. NBRF/PIR
- 13. NBRF/PIR
- 14. NBRF/PIR
- 15. SEQUENCE RETRIEVED FROM NBRF/PIR IN FASTA FILE FORMAT >F7VJQ1 APRIO_HUMAN Alternative prion protein [Homo sapiens] MEHWGQPIPGAGQPWRQPLPTSGRWWLGAASWWWLGAASWWWLGAAPWWWLGTASWWWL G SRRWHPQSVEQAE
- 16. PDB • The Protein Data Bank (PDB) archive is the single worldwide repository of information about the 3D structures of large biological molecules, including proteins and nucleic acids. • The PDB was established in 1971 at Brookhaven National Laboratory (BNL) under the leadership of Walter Hamilton and originally contained 7 structures. • In 1998, the Research Collaboratory for Structural Bioinformatics (RCSB) became responsible for the management of the PDB. • In 2003, the wwPDBwas formed to maintain a single PDB archive of macromolecular structural data that is freely and publicly available to the global community. • The RCSB PDB supports a website where visitors can perform simple and complex queries on the data, analyze, and visualize the results. • Members of wwPDB are: RCSBPDB(USA), PDBe (Europe) and PDBj (Japan), and Biological Magnetic Resonance Data Bank BMRB(USA). http://rcsb.org/pdb/
- 17. PDB
- 18. PDB
- 19. PDB
- 20. SCOP • The Structural Classification of Proteins (SCOP) database is a largely manual classification of protein structural domains based on similarities of their structures and amino acid sequences. • A motivation for this classification is to determine the evolutionary relationship between proteins. • Proteins with the same shapes but having little sequence or functional similarity are placed in different "superfamilies", and are assumed to have only a very distant common ancestor. • Proteins having the same shape and some similarity of sequence and/or function are placed in "families", and are assumed to have a closer common ancestor. • SCOP has been discontinued and the last official version of SCOP is 1.75. SCOP1.75 is also known as SCOP2. • SCOP2 offers two different ways for accessing data: SCOP2-browser, and SCOP2-graph. • SCOP2-browser allows navigation in a traditional way by browsing pages displaying the node information. • SCOP2-graph is a graph-based web tool for display and navigation. • The source of protein structures is the Protein Data Bank.
- 21. HIERARCHICAL STRUCTURE OF SCOP • The unit of classification of structure in SCOP is the protein domain. • The levels of SCOP are as follows. 1. Class: Types of folds, e.g., all α, all β, α/β, α+β, α&β, etc. 2. Fold: The different shapes of domains within a class, e.g., 2 helices; antiparallel hairpin, left-handed twist, etc. 3. Superfamily: The domains in a fold are grouped into superfamilies, which have at least a distant common ancestor. 4. Family: The domains in a superfamily are grouped into families, which have more recent common ancestor. 5. Protein domain: The domains in families are grouped into protein domains, which are essentially the same protein. 6. Species: The domains in "protein domains" are grouped according to species. 7. Domain: It is part of a protein. For simple proteins, it can be the entire protein. http://scop2.mrc-lmb.cam.ac.uk/
- 22. HIERARCHICAL STRUCTURE OF SCOP
- 23. OUTPUT OF SCOP
- 24. OUTPUT OF SCOP
- 25. CATH • The CATH (Class, Architecture, Topology, and Homologous superfamily) is a semi- automatic, hierarchical classification of protein domains. • CATH shares many broad features with its principal rival, SCOP. • The four main levels of the CATH hierarchy are as follows: • Class: the overall secondary-structure content of the domain. e.g., all α, all β, α/β, α+β, α&β, etc. • Architecture: high structural similarity but no evidence of homology. Equivalent to a fold in SCOP. • Topology: a large-scale grouping of topologies which share particular structural features • Homologous superfamily: indicative of a demonstrable evolutionary relationship. Equivalent to the superfamily level of SCOP. http://www.cathdb.info/
- 26. CATH
- 27. CATH
- 28. MOTIF • Motif is a search service provided by GenomeNet to search with a protein query sequence against Motif Libraries. • Supports several motif databases such as Prosite, BLOCKS, ProDom, Pfam, and PRINTS. • Allows you to search protein sequence libraries with your patterns. • Each residue must be separated with - (minus sign). • x represents any amino acids. • [DE] means either D or E. • {FWY} means any amino acids except for F, W and Y • A(2,3) means that A appears 2 to 3 times consecutively. • The pattern string must be terminated with . (period). For example, C-x-{C}-[DN]-x(2)-C-x(5)-C-C. • Generates a profile from a set of multiple aligned sequences using PFMake or HMMBuild, respectively.http://www.genome.jp/tools/motif/
- 29. MOTIF
- 31. PATTERN OF MATCHING MOTIF HITS
- 32. PFAM • The Pfam database is a large collection of protein families, each represented by multiple sequence alignments and hidden Markov models (HMMs). • Pfam version 27.0 was produced at the European Bioinformatics Institute using a sequence database called Pfamseq, which is based on UniProt. • The descriptions of Pfam families are managed by the general public using Wikipedia. • The Pfam database contains information about protein domains and families. • Pfam-A is the manually curated portion of the database that contains over 10,000 entries. • Pfam-B contains a large number of small families derived from clusters produced by an algorithm called ADDA (for automatic generation). • Pfam-B families can be useful when no Pfam-A families are found (but lower quality). http://pfam.xfam.org/
- 33. PFAM
- 34. PFAM
- 35. PROSITE • PROSITE, a protein domain database for functional characterization and annotation. • PROSITE consists of entries describing the protein families, domains and functional sites as well as amino acid patterns and profiles in them. • PROSITE is manually curated by a team of the Swiss Institute of Bioinformatics and tightly integrated into Swiss-Prot protein annotation. • PROSITE is complemented by ProRule, a collection of rules based on profiles and patterns. • The rules contain information about biologically meaningful residues, like active sites, substrate- or co-factor-binding sites, posttranslational modification sites or disulfide bonds, to help function determination. http://prosite.expasy.org/
- 36. PROSITE
- 37. PROSITE