PROTOPLAST: ISOLATION, FUSION & REGENERATION
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Non-ionic substances like mannitol and sorbitol as well as ionic substances like potassium chloride and calcium chloride are commonly used to maintain osmotic pressure in plant cell solutions. Potassium chloride, calcium chloride, and magnesium phosphate are ionic substances used, while mannitol is frequently the non-ionic substance chosen. The document also lists the specific quantities of potassium nitrate, calcium chloride, magnesium sulfate, potassium iodide, and copper sulfate that are made up to one liter of solution with a pH of 5.8.
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PROTOPLAST: ISOLATION, FUSION & REGENERATION
- 8. Non- Ionic Ionic Most commonly used are soluble carbohydrates such as mannitol, sorbitol, glucose, fructose, galactose and sucrose. Mannitol, being metabolically inert, is most. frequently used. Potassium chloride, calcium chloride and magnesium phosphate are the ionic substances in use to maintain osmotic pressure. KH2PO4 27.2mg KNO3 101mg CaCl2.H2O 1480mg MgSO4.7H2O 246mg KI 0.16mg CuSO4.5H2O 0.025mg Made to 1 litre with water pH 5.8