A PRELIMINARY ANALYSIS OF ALGAL
DIVERSITY OF SELECTED POND
IN KOZHIKODE DISTRICT, KERALA, INDIA.
Dr. Rajesh Kumar, Assistant Professor of
Botany, MGGAC, Mahe, Puducherry
INTRODUCTION
Algae are primitive photosynthetic organisms and they play an important role as
producers in aquatic ecosystem.
These are considered to be the expellers of huge volume of oxygen into the
atmosphere.
Algae have pigments like chlorophylls, carotenoids and phycobilins. Their
reserve food materials include starch and oils.
The mode of reproduction includes vegetative, asexual or sexual.
 Blue green algae are having the capability of Nitrogen fixation.
As a result of eutrophication, algal blooms may develop which may
deoxygenate water and cause the death of aquatic life.
In fresh water, algae are found as planktonic, benthic or epiphytic. The
planktonic algae can tolerate only low rate of water flow whereas benthic algae
can tolerate high flow rate.
Green and blue green algae are the major groups that cause, algal blooming.
STUDY AREAAND ITS MAP
MATERIALS AND METHODS
Sampling was done by using specific filter cloth (mesh size 25µm).
samples were collected, in 2 seasons – pre-monsoon and post monsoon.
In each collection, 30L of water was taken by using a sterilized container of
5L volume.
The water was drained out and debris containing algal samples were
transferred to sterilized plastic vials using pond water as medium.
The collected samples were brought to laboratory and kept in refrigerator.
On the following day, samples were taken, centrifuged in a sterilized
centrifuge tube for 15 min at 900 rpm
.
Supernatant was discarded and the debris was collected with the help of a
micropipette and kept on a clean glass slide with cover glass in watery medium
under high power of electric compound microscope.
The algae were recorded in the note book along with measurement using
micrometer.
The size, length, shape and colour of the chloroplast etc. were noted and algal
members were identified with the help of standard algal flora key like
Prescott(1948).
The algae were classified according to their classes.
SAMPLING SITE
Pre-monsoon Post monsoon
RESULTS
The analysis of algae showed a total of 17 species .
Of these 8 members belong to Cholorophyceae, 3 belongs
to Bacillariophyceae, 2 each of Xanthophyceae and
Cyanophyceae, 1 each of Dianophycae and Euglenophyeae.
Sl.
No.
SCIENTIFIC
NAME
CLASS DISTRIBUTION
SAMPLE 1 SAMPLE 2
1 Chlorella
pyrenoidosa
Chlorophyceae
(Colonial)
+ +
2 Closterium
closteroides
Chlorophyceae
(Unicellular)
- +
3 Closterium
kuetzingii
Chlorophyceae
(Unicellular)
+ +
4 Cosmarium
decoratum
Chlorophyceae
(Unicellular)
- +
5 Euglena
imnophilia
Euglenineae
(Unicellular)
- -
6 Melosira italica Bacillariophyceae
(Filamentous)
+ +
7 Micrasterias
foliacia
Chlorophyceae
(Filamentous)
+ +
8 Nostoc
punctiforme
Cyanophyceae
(Filamentous)
+ +
9 Ophiocytium
majus
Xanthophyceae (Colonial) + +
10 Oscillatotia irrigua Cyanophyceae
(Filamentous)
+ +
11 Oscillatoria princeps Cyanophyceae
(Filamentous)
+ +
12 Perdinum cinctum Dinophyceae
(Unicellular)
- +
13 Pinnularia crucifera var.
subcapitata
Bacillariophyceae
(Unicellular)
- -
14 Selanastrum gracile Chlorophyceae
(Colonial)
_ +
15 Spirogyra intorta Chlorophyceae
( Filamentous)
+ +
16 Tabellaria flocullosa Bacillariophyceae
(Filamentous)
+ -
17 Ulothrix zonata Chlorophyceae
(Filamentous)
_ _
 The seasonal diversity of algae showed much variation in case of both
seasons. The algae were easily distinguished in post monsoon, clumps
of many algae were observed in pre-monsoon, but were not in
distinguishable forms.
 Of the 17 algal species identified 7 members belongs to
Chlorophyceae, 4 belongs to Bacilariophyceae, 2 each of Xanthophyceae
and Cyanophyceae, 1 each of Dinophyceae and Euglenineae.
The dominant algal species occupied in the pond is Closterium spp. and
Oscillatoria spp.
47.05% is occupied by Chlorophycean members, 17.64% by
Bacillariophyceae, 11.76% by both Xanthophyceae and Cyanophyceae
and 0.58% is represented by Dinophyceae and Euglenuneae.
The pond has 7 unicellular (41.17%) , 3 colonial (17.64%) and 7
filamentous (41.17%).
 The analysis on the presence of algal members showed that 8
members (47.05%) were found In every 3 samplings, 17.64% were
found in 2 sampling and 35.29% of species were found in each
samplings.
algal members like Oscillatoria and Micrasterias etc in the
sampling was an indication of eutrophication , acidification and
metal contamination.
Selenastrum gracile Perdinum cinctum
Closterium closteroides
Pinnularia crucifera
var.subcapitata
Melosira italica
Ophiocytium majus Microsterias foliaceae
Oscillatoria princeps
Closterium kuetzingii
Ulothrix zonata
UNICELLULAR FILAMENTOUS COLONIAL
0
10
20
30
40
50
60
70
80
THALLUS ORGANIZATION OF ALGAE
THALLUS ORGANIZATION OF
ALGAE
18%
47%
12%
6%
12%
6%
ALGAL DIVERSITY OF SELECTED POND
Bacillariophyceae
Chlorophyceae
Cyanophyceae
Euglenaceae
Xanthophyceae
Dinophyceae
CONCLUSION
The study of algal diversity carried out in pond in Kuttiady region, is rich in
phytoplankton density anf find a suitable site for herbivorus fish farming. showed the
presence of 17 species of algae
These variations in distribution is due to the differences in temperature,
PH of water ,salinity etc.
Thank you

Rajesh PPT.pptx algal application and occurrence

  • 1.
    A PRELIMINARY ANALYSISOF ALGAL DIVERSITY OF SELECTED POND IN KOZHIKODE DISTRICT, KERALA, INDIA. Dr. Rajesh Kumar, Assistant Professor of Botany, MGGAC, Mahe, Puducherry
  • 2.
    INTRODUCTION Algae are primitivephotosynthetic organisms and they play an important role as producers in aquatic ecosystem. These are considered to be the expellers of huge volume of oxygen into the atmosphere. Algae have pigments like chlorophylls, carotenoids and phycobilins. Their reserve food materials include starch and oils. The mode of reproduction includes vegetative, asexual or sexual.  Blue green algae are having the capability of Nitrogen fixation. As a result of eutrophication, algal blooms may develop which may deoxygenate water and cause the death of aquatic life. In fresh water, algae are found as planktonic, benthic or epiphytic. The planktonic algae can tolerate only low rate of water flow whereas benthic algae can tolerate high flow rate. Green and blue green algae are the major groups that cause, algal blooming.
  • 3.
  • 4.
    MATERIALS AND METHODS Samplingwas done by using specific filter cloth (mesh size 25µm). samples were collected, in 2 seasons – pre-monsoon and post monsoon. In each collection, 30L of water was taken by using a sterilized container of 5L volume. The water was drained out and debris containing algal samples were transferred to sterilized plastic vials using pond water as medium. The collected samples were brought to laboratory and kept in refrigerator. On the following day, samples were taken, centrifuged in a sterilized centrifuge tube for 15 min at 900 rpm .
  • 5.
    Supernatant was discardedand the debris was collected with the help of a micropipette and kept on a clean glass slide with cover glass in watery medium under high power of electric compound microscope. The algae were recorded in the note book along with measurement using micrometer. The size, length, shape and colour of the chloroplast etc. were noted and algal members were identified with the help of standard algal flora key like Prescott(1948). The algae were classified according to their classes.
  • 6.
  • 7.
    RESULTS The analysis ofalgae showed a total of 17 species . Of these 8 members belong to Cholorophyceae, 3 belongs to Bacillariophyceae, 2 each of Xanthophyceae and Cyanophyceae, 1 each of Dianophycae and Euglenophyeae.
  • 8.
    Sl. No. SCIENTIFIC NAME CLASS DISTRIBUTION SAMPLE 1SAMPLE 2 1 Chlorella pyrenoidosa Chlorophyceae (Colonial) + + 2 Closterium closteroides Chlorophyceae (Unicellular) - + 3 Closterium kuetzingii Chlorophyceae (Unicellular) + + 4 Cosmarium decoratum Chlorophyceae (Unicellular) - + 5 Euglena imnophilia Euglenineae (Unicellular) - - 6 Melosira italica Bacillariophyceae (Filamentous) + + 7 Micrasterias foliacia Chlorophyceae (Filamentous) + + 8 Nostoc punctiforme Cyanophyceae (Filamentous) + + 9 Ophiocytium majus Xanthophyceae (Colonial) + +
  • 9.
    10 Oscillatotia irriguaCyanophyceae (Filamentous) + + 11 Oscillatoria princeps Cyanophyceae (Filamentous) + + 12 Perdinum cinctum Dinophyceae (Unicellular) - + 13 Pinnularia crucifera var. subcapitata Bacillariophyceae (Unicellular) - - 14 Selanastrum gracile Chlorophyceae (Colonial) _ + 15 Spirogyra intorta Chlorophyceae ( Filamentous) + + 16 Tabellaria flocullosa Bacillariophyceae (Filamentous) + - 17 Ulothrix zonata Chlorophyceae (Filamentous) _ _
  • 10.
     The seasonaldiversity of algae showed much variation in case of both seasons. The algae were easily distinguished in post monsoon, clumps of many algae were observed in pre-monsoon, but were not in distinguishable forms.  Of the 17 algal species identified 7 members belongs to Chlorophyceae, 4 belongs to Bacilariophyceae, 2 each of Xanthophyceae and Cyanophyceae, 1 each of Dinophyceae and Euglenineae. The dominant algal species occupied in the pond is Closterium spp. and Oscillatoria spp. 47.05% is occupied by Chlorophycean members, 17.64% by Bacillariophyceae, 11.76% by both Xanthophyceae and Cyanophyceae and 0.58% is represented by Dinophyceae and Euglenuneae.
  • 11.
    The pond has7 unicellular (41.17%) , 3 colonial (17.64%) and 7 filamentous (41.17%).  The analysis on the presence of algal members showed that 8 members (47.05%) were found In every 3 samplings, 17.64% were found in 2 sampling and 35.29% of species were found in each samplings. algal members like Oscillatoria and Micrasterias etc in the sampling was an indication of eutrophication , acidification and metal contamination.
  • 12.
    Selenastrum gracile Perdinumcinctum Closterium closteroides Pinnularia crucifera var.subcapitata
  • 13.
    Melosira italica Ophiocytium majusMicrosterias foliaceae Oscillatoria princeps
  • 14.
  • 15.
    UNICELLULAR FILAMENTOUS COLONIAL 0 10 20 30 40 50 60 70 80 THALLUSORGANIZATION OF ALGAE THALLUS ORGANIZATION OF ALGAE
  • 16.
    18% 47% 12% 6% 12% 6% ALGAL DIVERSITY OFSELECTED POND Bacillariophyceae Chlorophyceae Cyanophyceae Euglenaceae Xanthophyceae Dinophyceae
  • 17.
    CONCLUSION The study ofalgal diversity carried out in pond in Kuttiady region, is rich in phytoplankton density anf find a suitable site for herbivorus fish farming. showed the presence of 17 species of algae These variations in distribution is due to the differences in temperature, PH of water ,salinity etc.
  • 18.