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NUCLEUS
NUCLEUS
• Controls –cellular activities
• Controlling centre
• Robert brown-1831
• Karyology
• +all Eukaryotes
• - RBC,Lence cells of eye
NUCLEUS
• Cell-1 nucleus-2 or more
• Types of cells based on no of nucleus
1. Mononucleate- Amoeba, typical cell
2. Binucleate-Paramecium,cartilage,liver
3. Polynucleate-Opalina
NUCLEUS
• Shape:
–Shape of the cell
–Generally Spherical
• Chemical nature:
–80%-Proteins
–12% DNA
–5%RNA
–3%Lipids
NUCLEUS
Size
• Not constant-cell type
• 10% cell volume
• 3μm -25 μm
• Size directly proportional to cytoplasm
• More volume-larger nucleus
NUCLEUS
R.HERTWIG
Volume of nucleus
(NP) Volume of cytoplasm-volume of nucleus
Vn
Vc-Vn
Vn =Volume of nucleus
Vc =Volume of Cytoplasm
Nucleo cytoplasmic index =
(NP)=
NUCLEUS
• Structure
1. Nuclear membrane
2. Nucleoplasm
3. Chromatin reticulam
4. nucleolus
NUCLEAR MEMBRANE
 Semipermiable membrane
 Double layer-Outer, inner layer
 Perinuclear space(10-15 nm)
 Minute pores-circular-exchange
 Outer rough,inner smooth
Outer inner layer
NUCLEAR SAP
• Nucleoplasm/karyolymph
• Transparent,semi-solid,acidophilic
• Chromatin,nucleolus-suspended
• Organic,inorganic-Nucleic
acids,Proteins,Enzymes,minerals
CHROMATIN RETICULAM
• Thread like structures- Chromonemata
• Net work-Chromatin reticulam
• Cell division-chromatin-ribbon like
chromosomes
• Chemically-DNA,Proteins
• 2 types
1. Euchromatin
2. Heterochromatin
CHROMATIN RETICULAM
1. Hetero chromatin
• Darkly stain,condensed
• Small-DNA,Large RNA
2.EuChromatin
Light stained,diffused
Large DNA,Small RNA
4.NUCLEOLUS
• Discovered- Fontana
• Coined –Bowman
• Distinct in interphase
• Disappear-Pro,meta,ana phase
• Reappear telophase
• Non membrane
• Number depends on chromosome sets
• Composed –RNA ,Proteins
• Function-rRNA,Prorein
FUNCTIONS
• Control cellular activities
• Master plan for protein
• Nucleolus-Ribosomes
• rRNA synthesis
• Reg-cell cycle
• Heredity

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STRUCTURE AND FUNCTIONS OF NUCLEUS & NUCLEOLUS