Substrate level phosphorylation and it's mechanism || Biochemistry || B Pharmacy || Project || slideshare || biology ||

Substrate level phosphorylation and it's mechanism || Biochemistry || B Pharmacy || Project || slideshare || biology ||

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  Substrate Level PhosphorylationAnd Its Mechanism Name : Darshan Ratan Jadhav Class : F.Y.B.Pharmacy Subject : Biochemistry College : K.K.Wagh College of Pharmacy, Nashik
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  Content •Introduction •Example •Reaction •Reference Content • Introduction • Mechanism •The role of substrate-level phosphorylation in aerobic respiration. • Refference
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  Substrate Level Phosphorylation ➢Phosphorylation involves the transfer of phosphate group from one compound to other. ➢ Substrate level phosphorylation is a direct phosphorylation of ADP with a phosphatase group by using the energy obtain from a coupled reaction. ➢ Occurs in cytoplasm ( glycolysis – due to aerobic and anaerobic condition) and in mitochondrial matrix ( krebs cycle – anaerobic condition)
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  Genral Substrate Level PhosphorylationReaction
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  Mechanism of Substratelevel phosphorylation • High-energy phosphate groups are transferred directly from phosphorylated substrates to ADP • ATP is synthesized via substrate-level phosphorylation in glycolysis and the Krebs cycle
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  Catalysis
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  The role ofsubstrate-level phosphorylation in aerobic respiration • Substrate-level Phosphorylation and Aerobic Respiration: • We know that there is the formation of 32 ATP molecules in total (since 2 ATP molecules are used in the beginning of glycolysis the final ATP yield is 30) as a result of the complete oxidation of one glucose molecule in aerobic respiration. • Out of all 32 ATP molecules, 26 are produced by way of oxidative phosphorylation with the involvement of NADH and FADH2, whereas the remaining 6 are the result of substrate- level phosphorylation.
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  1. During glycolysis,the enzyme phosphoglycerate kinase transfers the high energy phosphoryal group from the carboxyl group of 1,3-bisphosphoglycerate to ADP, forming ATP and 3-phosphoglycerate.
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  during glycolysis, theenzyme pyruvate kinase transfers the high energy phosphoryl group from phosphoenolpyruvate to ADP, forming ATP and pyruvate. 2. ➢ In glycolysis during substrate level phosphorylation 4 ATP molecules are produce.
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  During TCA cycle(Citric acid cycle; Kreb’s cycle), the enzyme succinyl-CoA synthetase breaks the thioester bond of succinyl-CoA andthe energy released in this breakage is used to drive the synthesis of a phosphoanhydride bond in GTP or ATP. Succinate is the product in the process. 3. ➢ In This Step 2 ATP is produce
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  Refference • Biochemistry BookBy U. Satyanarayana & U. Chakrapani. • www.google.com • Guidance of subject teacher