This document presents a mathematical model to analyze the effect of imbalance on the performance of an unpaced five-station production line. The model considers different operation time distributions across stations with no buffer capacities. It evaluates 60 assignments of the five operations to stations and calculates the production rate for each. The percent improvement in production rate between the best and worst assignments is used to determine the effect of imbalance. Simulation results show the effect is highly significant at moderate levels of imbalance. The model provides a way to optimize production rate by judiciously selecting operation assignments to stations for imbalanced lines.