Types of cell culture
- 1. TYPES CELL CULTURE PRESENTED By: Nadia
- 2. TYPES OF CELL CULTURE Cell culture is classified into three: i. Primary cell culture • Adherent cell culture • Suspension cell culture ii. Secondary cell culture iii. Cell line • Finite cell line • Continuous cell line
- 3. TYPES OF CELL CULTURE Cell culture is classified into three: i. Primary cell culture • Adherent cell culture • Suspension cell culture ii. Secondary cell culture iii. Cell line • Finite cell line • Continuous cell line
- 4. PRIMARY CELL CULTURE Separate cell directly from the parent tissue ( kidney, liver, heart etc.) Separated by enzymatic or mechanical method. Maintaining the growth of the cell in a culture medium using glass or plastic container. Culture medium o Liquid or gel designed to maintain the growth of the cell. o It varies from different type of cell.
- 5. PRIMARY CELL CULTURE Primary cell culture is further classified based on the type of cell: i. Adherent cell : • Those cell which attach to the surface of the culture flask. • Forms a monolayer. • They have to be detached from the surface before they get sub cultured. • Growth limited to surface area. ii. Suspension cell • Those cell does not get attached to the surface of culture flask. • They are free floating. • Cells in blood stream. • Growth is limited to concentration of cells.
- 6. SECONDARY CELL CULTURE When primary cell is sub cultured then it is secondary cell. Sub culture; Transfer of cell from one culture vessel to another. Method: o Remove growth medium. o Detach adherent cells (enzymatic method) Why required: o To provide fresh nutrients. o To provide more space.
- 7. CELL LINE Finite cell lines Limited life span Go through a limited number of cell generation Properties: o Contact inhibition o Density limitation o Anchorage dependence Less growth rate Doubling time (24-92hrs.) Continuous cell lines Grow indefinitely Grows either in monolayer or in suspension Properties: o Absence Contact inhibition o Absence Anchorage dependence High growth rate Doubling time (12-24hrs.)