This document provides an overview and introduction to using the command line interface and submitting jobs to the NIAID High Performance Computing (HPC) Cluster. The objectives are to learn basic Unix commands, practice file manipulation from the command line, and submit a job to the HPC cluster. The document covers topics such as the anatomy of the terminal, navigating directories, common commands, tips for using the command line more efficiently, accessing and mounting drives on the HPC cluster, and an overview of the cluster queue system.