Water in hplc 2007

Water in hplc 2007

• 1.
  Water in HPLCUnderthe Supervision of: PROF.DR. Sawsan ElmasryPresented by: Reem Tawfeek
• 2.
  Points to bediscussedWater used in HPLC.Lab water grades.Specifications of HPLC-grade water. how to determine water purity?Problems caused by poor quality water.Package and storage.Conclusion.
• 3.
  Water usedin HPLCWater for HPLC may be prepared IN HOUSE (analytical labs may prepare its own HPLC water)(N.B. that what most frequently happens in Egyptian labs)Freshly prepared double distilled water (within 24 hours) will be convenient for HPLC applications
• 4.
  Water usedin HPLCDe-ionized water will be INCONVENIENT for HPLC application due to uncertainty of the absence of the microbial contaminationOR labs could obtain water from HPLC-grade chemicals supplie
• 5.
  Lab Water Grades
• 6.
  the different waterspecifications based on the different water types
• 7.
  HPLC-water specificationsAccording toB.P. 2010 water for chromatography isdeionized water with a resistivity of not less than 18 MΩ-cmBut is this enoughIt’s possible to have 18 MΩ-cm soup! Can we use soup for HPLC?????
• 8.
  How to measurewater purity? contaminants in water either
• 9.
  Sources of organiccontaminants [Total organic carbon(TOC)]Leaching from purification media, tubing and containers,bacterial contaminationabsorption from the atmosphere
• 10.
  Poor quality waterreduces chromatographic performance byaffecting resolution and integration.introducing ghost peaks.altering stationary phase selectivity.and impacting baselines.
• 11.
  Ghost peaks
• 12.
  Isocratic baseline monitoringat 210 nm using 100% high TOC reagent water(10 Mᾩ·cm resistivity, 100 ppb TOC) (1 mL/min).
• 13.
  Using high-purity water(3ppb TOC)
• 14.
  According to AmericanSociety for Testing and Materials ASTM Reagent grade water should containsTotal organic carbon (TOC), max , 50 ppbResistivity not less than 18MΩ-cm
• 15.
  Packing and storagepackagedin amber glass bottles sealed under a nitrogen with Teflon-lined fluorocarbon caps .Unless other instructions stated by SOP(standard operating procedures) Once the bottle is opened it should be used within 24 hours.
• 16.
  Conclusion We shouldswitch from simple resistivity measurement to resistivity and TOC monitoring in order to be able to take reagent water quality for granted.
• 17.
  referencesW.M.A. Niessen, J.Chromatogr., A 856(1,2),179–197 (1999).W. Byrne, Reverse Osmosis — A Practical Guide for Industrial Users. 2nd ed. (Tall Oaks Publishing,Littleton, Colorado, 2002).Standard Specification for Reagent Water American Society for Testing and Materials International 2001 Annual Book of Standards – Volume 11.01B. Srikanth, Ultrapure Water 15(3), 40–46(1998).K. Clark, M. Retzik, and D. Darbouret, Ultrapure Water 14(2), 21–24, (1997).C. Regnault, I. Kano, D. Darbouret, and S.Mabic,J. Chromatogr., A 1030(1–2), 289–295(2004).B. Stewart and B. Williamson, Am. Biotechnol. Lab., 16–18, December 2001. http://www.high-q.com/pdf/q&a_reagent_water_screen.pdf