Cell counting
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This document describes how to use a hemocytometer, an instrument used to count blood cells. It includes a Neubauer slide with a central counting area divided into squares, a cover slip, and pipettes. The process involves placing a blood sample on the slide, focusing it under a microscope, and counting the cells within the squares to calculate cell density using the number of squares and an equation.
Cell counting
- 1. Ariola, Rhoanna L. Diamance, Mary Hazel B.
- 2. Hemo: blood Cyto: cell Meter: measurement/counter *Thus, it is an instrument used to count the blood cells.
- 3. It includes: a) Neubauer’s slide b) Cover slip c) micropipette d) RBC pipette e) WBC pipette
- 4. It is a thick glass slide, center of which has double ruling area separated by troughs (these four troughs are extending across the slide and set parallel to each other. The fifth one is separating the two ruling areas from each other).
- 5. Step 1. Put the glass cover on the Neubauer slide central area. Take 10 µl sample from a suspension using a micropipette. Introduce it to the Neubauer chamber. When the pipette is loaded, it must always be held in vertical position. Place pipette tip close to the glass cover edge, right at the centre of the Neubauer chamber. Release the plunger slowly watching how the liquid enters the chamber uniformly, being absorbed by capillarity. In case of the appearance of bubbles, or that the glass cover has moved, repeat the operation.
- 7. Step 2. Place the Neubauer chamber on the microscope stage. Turn on the microscope light.. Focus the microscope until a sharp image of the cells is already seen. Look for the first counting grid square where the cell count will start.
- 8. 1 mm 0.04 mm
- 11. Cell density is ….. For bigger squares: (A+B+C+D)(2500)
- 12. a b c d e