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protein structure guy

@hellsite-proteins

i can turn your favourite posts into neat shapes :)

Hi!

the goal of this blog is to use the strings of letters from text posts matching proteinogenic amino acids into 3D structures using AlphaFold. i am not affiliated with AlphaFold in any way, just using it to create and share some neat images. the idea for this was heavily inspired by @hellsitegenetics .

to make these, i will be removing any letters not matching proteinogenic amino acids, as well as any other characters, and plugging the resulting sequence into AlphaFold3. while i tried in some early posts, unusual amino acids unfortunately do not work in my structures. therefore, B, J, O, U, X and Z will all be omitted. in the final image i will usually only be showing the cartoon and hide the surface and side chains, for the sake of making a somewhat more pleasant image. i now use ChimeraX to open and show structures. they may not always be accurate depending on the string of letters, so unless i say anything else, assume that none of it is reliable!

please send me any posts you want to see made into structures :) i can't promise i'll answer fast but i will do my best

some of the more popular protein memes:

here are some tags i tend to use to categorize things:

#protein asks for inbox submissions

#protein memes and #protein songs for memes and songs

#protein info if i'm answering science questions or explaining things

lets hear it for transgenderism and faggotry. can I get a round of applause for transgenderism and faggotry

also shoutout to queerness and dykishness and gender fuckery. if you reblog this post you hate conservatives

woohoo!!

letter sequence in this post matching protein-coding amino acids:

letshearitfrtransgenderismandfaggtrycanIgetarndfapplasefrtransgenderismandfaggtryalsshtttqeernessanddykishnessandgenderfckeryifyrelgthispstyhatecnservatives

protein guy analysis:

we have a couple beta sheets, which suggest that enough of this protein is working together to at least give us an extended secondary structure. we love to see the collaboration and unity here! the rest of the structure is more scattered, with loops set up fairly far apart and likely disordered. of course, none of this is reliable and the confidence is nearly non-existent, but we can always hope

predicted protein structure:

how many of the protiens on this blog actually turn out to be Wretched Things

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aside from the very few times i've done a real protein, the answer is somewhere between most and all of them :)

letter sequence in this ask matching protein-coding amino acids:

hwmanyftheprtiensnthislgactallytrntteWretchedThings

protein guy analysis:

this is a neat looking one, and is essentially all beta sheet! what's more, the confidence is slightly less bad and usual. of course, that still lands in the 50-70% range, and it would still be unreasonably optimistic to fully believe in the backbone that has been generated, but its still very cool to look at.

predicted protein structure:

cartoon coloured by pLDDT

cell bio professor closed out today's lecture on free-radical oxidation in mitochondria and programmed cell death by saying "you've probably all seen those commercials for fruit juice that says it's got antioxidants, which are said to prevent this sort of thing from happening, or at least slow it down. well, they don't work. this is an inevitable fact of life— this process that lets us live is also the thing that kills us, and it's why all of us will die someday. there's no escaping that. it's been with us since the dawn of eukaryotic cells; our pact with mitochondria is to the death. anyway, enjoy the rest of your friday, and remember, exam four is next week!"

one day, entropy will win and you will end

biology prof existentialism is one of the most beautiful and comforting things to me, but that may just be my own personal bias

also, for some reason the wording of this reminds me of the time my ochem prof closed with something along the lines of "...and that's how you make fentanyl. it's a wonderful drug. have a nice reading break!"

letter sequence in this post matching protein-coding amino acids:

celliprfessrclsedttdayslectrenfreeradicalidatininmitchndriaandprgrammedcelldeathysayingyvepralyallseenthsecmmercialsfrfriticethatsaysitsgtantiidantswhicharesaidtpreventthissrtfthingfrmhappeningratleastslwitdwnwelltheydntwrkthisisaninevitalefactflifethisprcessthatletssliveisalsthethingthatkillssanditswhyallfswilldiesmedaytheresnescapingthatitseenwithssincethedawnfekaryticcellsrpactwithmitchndriaistthedeathanywayenytherestfyrfridayandrememereamfrisnetweek

protein guy analysis:

this one has a nice mix of helices and sheets, surrounded by so many loops. it's not a good structure, and the confidence is low enough that i wouldn't derive any meaning from it, but i'm sure many of you will choose to do so anyways. this isn't a judgement, and if it brings you joy then that is meaning enough to do anything

predicted protein structure:

Anonymous asked:

MWELRSIAFSRAVFAEFLATLLFVFFGLGSALNWPQALPSVLQIAMAFGLGIGTLVQALGHISGAHINPAVTVACLVGCHVSVLRAAFYVAAQLLGAVAGAALLHEITPADIRGDLAVNALSNSTTAGQAVTVELFLTLQLVLCIFASTDERRGENPGTPALSIGFSVALGHLLGIHYTGCSMNPARSLAPAVVTGKFDDHWVFWIGPLVGAILGSLLYNYVLFPPAKSLSERLAVLKGLEPDTDWEEREVRRRQSVELHSPQSLPRGTKA

(I hope this works)

oh sick its an aquaporin!! this is a membrane channel protein that selectively allows water to pass in and out of cells, and is important for your kidneys to function, among other things. i don't know a ton of these off hand, but i think membrane proteins are very cool and i'm always a little blown away by the selectivity of different channel proteins.

letter sequence in this ask matching protein-coding amino acids:

MWELRSIAFSRAVFAEFLATLLFVFFGLGSALNWPQALPSVLQIAMAFGLGIGTLVQALGHISGAHINPAVTVACLVGCHVSVLRAAFYVAAQLLGAVAGAALLHEITPADIRGDLAVNALSNSTTAGQAVTVELFLTLQLVLCIFASTDERRGENPGTPALSIGFSVALGHLLGIHYTGCSMNPARSLAPAVVTGKFDDHWVFWIGPLVGAILGSLLYNYVLFPPAKSLSERLAVLKGLEPDTDWEEREVRRRQSVELHSPQSLPRGTKA

protein guy analysis:

this structure has a really high confidence everywhere except one helix. i've found while trying to use alpha fold to look at mutants in my protein of interest that some areas consistently have lower confidence even though a full structure is solved, although in my case that is probably because the area with lower confidence is less structured and interacts with other subunits while i have only tried to predict the monomeric form. still, it's very nice to see something that generally works, and that looks like a real protein (because it is!). overall, very neat and i'm happy to see it

predicted protein structure:

cartoon coloured by pLDDT

Met my neighbour's dog today. Fucked up little chihuahua that they let out on their front lawn offleash and forget about. Buuuut I have a hard time sympathizing with the fact that he is clearly fucked up for a reason because I keep finding little nasty nasty NASTY shits in front of my entry gate that are clearly his.

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gross!

letter sequence in this ask matching protein-coding amino acids:

MetmyneighrsdgtdayFckedplittlechihahathattheylettntheirfrntlawnffleashandfrgetattIhaveahardtimesympathiingwiththefactthatheisclearlyfckedpfrareasnecaseIkeepfindinglittlenastynastyNASTYshitsinfrntfmyentrygatethatareclearlyhis

protein guy analysis:

this one is mostly alpha helical, but the helices don't seem to want anything to do with each other since there is too much space between them for me to believe in a tertiary structure. there is also a weirdly twisted beta sheet, which can only partially be attributed to some extra flexibility from a glycine. it's not the worst on here by any means, but this peptonomination, like pretty much everything else on this blog, is clearly unwell

predicted protein structure:

We ought to start having an n/10 scale or something on how likely a protein is to give you a prion-related disease. It would definitely add a bit of entertainment, at least.

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i have absolutely no idea how we could calculate that, but i can say that the amyloid-looking beta sheet monstrosities i've gotten recently would be getting very high scores because i hate them

letter sequence in this ask matching protein-coding amino acids:

WeghttstarthavingannscalersmethingnhwlikelyaprteinistgiveyaprinrelateddiseaseItwlddefinitelyaddaitfentertainmentatleast

protein guy analysis:

this one also has beta sheets, but they are nowhere near as bad. this is a small but long-ish pair of beta sheets, followed by a disordered C-terminal tail. the sheet twists a little bit, which is actually fairly common for beta sheets in real structures. overall, this does seem like something that could conceivably fold, but given the confidence scores i still wouldn't assume that it really tells us anything reliable

predicted protein structure:

when talking about AI remember the different versions:

Analytical AI, is the one that can detect cancer and save lives

Generative AI is the one that steals art to make it worse, and gives you a wrong answer every time you google something

Weird Al is the one who got his ponysona to canonically have children with a pony from my little pony

very good summary!

letter sequence in this post matching protein-coding amino acids:

whentalkingatAIrememerthedifferentversinsAnalyticalAIisthenethatcandetectcancerandsavelivesGenerativeAIisthenethatstealsarttmakeitwrseandgivesyawrnganswereverytimeygglesmethingWeirdAlisthenewhgthispnysnatcannicallyhavechildrenwithapnyfrmmylittlepny

protein guy analysis:

turns out there is one more type of AI and that is the desperate hallucinations of a computer trying its best – which is apparently my specialty here. this one has a mix of helices and beta sheets, but none of them are reliable enough to be worth interpreting. i guess it does look cool though, if that counts for anything?

predicted protein structure:

cartoon coloured by pLDDT

Anonymous asked:

idk if this is a fair ask cause it's about a real peptide but. I saw your post about the 6 aa peptide and was wondering if alphafold can show a 5 aa peptide. There's the Extracellular Death Factor (EDF) which is NNWNN and I really wanted to see how it looked like and what you thought of it based on the structure alone

very cool! i know peptides don't make good drugs, but something about a molecule small enough that it makes sense to look at its chemical structure feels to me like it would be used that way (or maybe i've just taken too many classes with units about drug design?)

AF3 was able to make something for this little structure, but i'm not sure how useful it is

letter sequence in this ask matching protein-coding amino acids:

NNWNN

protein guy analysis:

this is small enough that ChimeraX initially opens it as a stick structure and not a cartoon, which makes sense because the cartoon is pretty underwhelming. it is too small to really make any secondary structure, which does also mean that the predicted confidence is incredibly high. overall, this is just a little guy and its very neat :)

predicted protein structure:

stick representation

cartoon representation

Anonymous asked:

what if i just PGPGPGPGPGPGPGGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPPGPGPGPGPGGPGPGPGPGPGPG

sure, why not? i see nothing wrong with this perfectly normal, naturally evolved sequence

letter sequence in this ask matching protein-coding amino acids:

whatifistPGPGPGPGPGPGPGGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPPGPGPGPGPGGPGPGPGPGPGPG

protein guy analysis:

this looks about as weird as i would expect for a combination of the two biggest exceptions to normal allowed ramachandran angles. it's all twists, but not twists that form a standard helical structure. there are also a couple sharp hairpin turns, which make sense given that proline and glycine are commonly used to make these sorts of turns. the confidence is still very low, but it's a bit better than a lot of other structures, which i think is very neat.

predicted protein structure:

cartoon, coloured by pLDDT

cartoon with side chains shown

pLDDT colour code

anomalocaris is a maid

statements that would make absolutely no sense on any other website

she has frills and stuff

This post is: Kiki

sure thing!

letter sequence in this ask matching protein-coding amino acids:

anmalcarisisamaidstatementsthatwldmakeasltelynsensenanytherwesiteshehasfrillsandstffThispstisKikihellsitegeneticsandnthatsamepsthellsiteprteins

protein guy analysis:

i really like how this structure looks. i can't explain why, but it feels like just a little guy here. i really like the slightly twisted beta sheets, which feel like something you could easily see in a real polypeptide. there is still a fair bit of disorder, which i'm not mad at. of course, the confidence is still abysmal but i've stopped really believing in these things and just looking for vibes :)

predicted protein structure:

Hełło

If is tower of Babel in Tagalog, because Tagalog makes anything better! I hopes it will not be too long.

At ang buong lupa ay iisa ang wika at iisa ang salita.

At nangyari, na, sa kanilang paglalakbay sa silanganan, ay nakasumpong sila ng isang kapatagan sa lupain ng Sinar; at sila'y nanahan doon.At nagsangusapang, Halikayo! tayo'y gumawa ng mga laryo, at ating lutuing mabuti. At inari nilang bato ang laryo at ang betun ay inaring argamasa.

At nagsipagsabi, Halikayo! Magtayo tayo ng isang bayan natin at ng isang moog, na ang taluktok niyaon ay aabot hanggang sa langit, at magtaglay tayo ng isang ngalan; baka tayo'y mangalat sa ibabaw ng buong lupa.

At bumaba ang Panginoon upang tingnan ang bayan at ang moog, na itinayo ng mga anak ng mga tao.

At sinabi ng Panginoon, Narito, sila'y iisang bayan at silang lahat ay may isang wika; at ito ang kanilang pinasimulang gawin: at, ngayon nga'y walang makasasawata sa anomang kanilang balaking gawin.

Halikayo! tayo'y bumaba at diyan din ay ating guluhin ang kanilang wika, na anopa't sila'y huwag magkatalastasan sa kanilang salita.

Ganito pinanabog sila ng Panginoon mula riyan, sa ibabaw ng buong lupa; at kanilang iniwan ang pagtatayo ng bayan.

Kaya ang pangalang itinawag ay Babel; sapagka't doon ginulo ng Panginoon ang wika ng buong lupa: at mula roon ay pinanabog sila ng Panginoon sa ibabaw ng buong lupa.

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neat! i'm excited to see how this one turns out. it's always cool to look at things in other languages

letter sequence in this ask matching protein-coding amino acids:

AtangnglpaayiisaangwikaatiisaangsalitaAtnangyarinasakanilangpaglalakaysasilangananaynakasmpngsilangisangkapatagansalpainngSinaratsilaynanahandnAtnagsangsapangHalikaytayygmawangmgalaryatatingltingmatiAtinarinilangatanglaryatangetnayinaringargamasaAtnagsipagsaiHalikayMagtaytayngisangayannatinatngisangmgnaangtalktkniyanayaathanggangsalangitatmagtaglaytayngisangngalanakatayymangalatsaiaawngnglpaAtmaaangPanginnpangtingnanangayanatangmgnaitinayngmgaanakngmgataAtsinaingPanginnNaritsilayiisangayanatsilanglahataymayisangwikaatitangkanilangpinasimlanggawinatngaynngaywalangmakasasawatasaanmangkanilangalakinggawinHalikaytayymaaatdiyandinayatingglhinangkanilangwikanaanpatsilayhwagmagkatalastasansakanilangsalitaGanitpinanagsilangPanginnmlariyansaiaawngnglpaatkanilanginiwanangpagtatayngayanKayaangpangalangitinawagayaelsapagkatdnginlngPanginnangwikangnglpaatmlarnaypinanagsilangPanginnsaiaawngnglpa

protein guy analysis:

oh no! this isn't good! i'm so sorry to whoever asked this but it looks terrible. it's like if a caterpillar hated you and wanted to inflict a slow and painful death upon you. as always, the one consolation is that the confidence is incredibly low.

predicted protein structure:

Anonymous asked:

the muppet joker's DNI

Greetings, Homosapians.

My name? Heh. Don't be so coy.

Kermit. Croaker. Joker. Clown Prince of Crime. Bro Strider. The Original Adam. Vriska Serket. I am a man of many names, so keep up unless you want to be schooled by myself or my league of dedicated followers. Mess with Croaker Nation if you dare. Don't ask me about my Pro Nouns unless you want me to roll my eyes at you and kick you. HARD. I train my kicks for hours every day, so bones WILL be broken.

♤♡◇♧

DNI:

  • Other Kermit/Joker kintypes
  • People who frequently post about Dennys (I have Dennys trauma. Genuinely fuck all of you Dennys enjoyers! Toxic.)
  • Anyone who kins Gonzo or Batman. Fuck you, Batsy... *voice drops to a low growl* And double fuck you, Gonzo.
  • Protestants. (Self Explanatory)
  • Klance shippers-- annoying.
  • Brennan Lee Mulligan apologists.
  • People under 21, just a personal preference, please respect it
  • Anyone who engages with Harry Potter-- fuck terfs and fuck JKR!
  • Snape apologists
  • Anyone who violates my previous DNI. DO YOUR OWN RESEARCH AND RESPECT MY BOUNDARIES. It is not my job to do emotional labor for you and tell you what those boundaries are. Be respectful and figure it out.
  • One exception to my previous DNI, however: Women may interact with my blog. You see, I have been reading a webcomic called Homestuck and awakened a Vriska kintype in the process. It has made me realize how close minded I have been and that hating an entire gender based on shallow stereotypes is reductive and harmful.
  • Men DNI. I am an advocate for the feminist movement and have made it my sworn duty to destroy those who oppose it.
  • Good Omens fans. Y'all are annoying.
  • Tumblr user Strange Aeons. DO NOT INTERACT or I will get you with my sword. Consider yourself warned.

As for those of you who are afraid of seeing what real darkness and depravity looks like? Heh........

Run While You Can.

this ask is old enough that i don't think this DNI is entirely up to date (so sorry it's taken this many months to answer!!). its also got a very specific energy to it that i can't quite name

letter sequence in this ask matching protein-coding amino acids:

GreetingsHmsapiansMynameHehDntescyKermitCrakerkerClwnPrincefCrimerStriderTheriginalAdamVriskaSerketIamamanfmanynamesskeeppnlessywantteschledymyselfrmyleagefdedicatedfllwersMesswithCrakerNatinifydareDntaskmeatmyPrNnsnlessywantmetrllmyeyesatyandkickyHARDItrainmykicksfrhrseverydaysnesWILLerkenDNItherKermitkerkintypesPeplewhfreqentlypstatDennysIhaveDennystramaGeninelyfckallfyDennysenyersTicAnynewhkinsGnratmanFckyatsyvicedrpstalwgrwlAnddlefckyGnPrtestantsSelfEplanatryKlanceshippersannyingrennanLeeMlliganaplgistsPeplenderstapersnalpreferencepleaserespectitAnynewhengageswithHarryPtterfckterfsandfckKRSnapeaplgistsAnynewhvilatesmyprevisDNIDYRWNRESEARCHANDRESPECTMYNDARIESItisntmytdemtinallarfryandtellywhatthsendariesareerespectflandfigreittneeceptintmyprevisDNIhweverWmenmayinteractwithmylgYseeIhaveeenreadingawecmiccalledHmestckandawakenedaVriskakintypeintheprcessIthasmademerealiehwclsemindedIhaveeenandthathatinganentiregenderasednshallwsteretypesisredctiveandharmflMenDNIIamanadvcatefrthefeministmvementandhavemadeitmyswrndtytdestrythsewhppseitGdmensfansYallareannyingTmlrserStrangeAensDNTINTERACTrIwillgetywithmyswrdCnsideryrselfwarnedAsfrthsefywhareafraidfseeingwhatrealdarknessanddepravitylkslikeHehRnWhileYCan

protein guy analysis:

it looks like we're bringing back a hellsite proteins classic on this one with long disordered loops wrapping halfway around the protein. the rest of this peptonomination that does have a secondary structure is mostly alpha helical, but with one small beta sheet towards the middle. this whole thing looks incredibly unstable, and i'm not a fan.

predicted protein structure:

let's stick with the meme-able movie theme for a minute here

letter sequence in this ask matching protein-coding amino acids:

lrdfarqaad

protein guy analysis:

fittingly, this one is very short. it's also very weird and feels a lot to me like a fairy tale character. to put it simply, this one decided to cyclize itself. interestingly, it doesn't look like there was a peptide bond formed between the terminal amino and carboxyl groups, but rather that they're just hanging out next to each other. way too close i might add. despite this, the structure has a very high confidence score (>90%), but given how little there is to work with, and my general understanding of chemistry, i still don't really believe it. i'd love to hear everyone else's thoughts on this whole situation though!!

predicted protein structure:

stick structure, with N terminal lysine in green and C terminal aspartate in purple

cartoon structure with side chains hidden, coloured by pLDDT

Okay here's the full transcript of the Bee Movie, with all non-letter characters plus B, J, O, U, X and Z all removed, split into blocks of 4096 characters each because tumblr asks limit each block to 4096 characters. It was easy to clean out the invalid characters with a quick regex match, and surprisingly, it all fits into one ask. I am sorry.

AccrdingtallknwnlawsfaviatinthereisnwayaeeshldealetflyItswingsaretsmalltgetitsfatlittledyffthegrndTheeefcrsefliesanywayecaseeesdntcarewhathmansthinkisimpssileYellwlackYellwlackYellwlackYellwlackhlackandyellwLetsshakeitpalittlearryreakfastisreadyCmingHangnasecndHellarryAdamCanyelievethisishappeningIcantIllpickypLkingsharpsethestairsYrfatherpaidgdmneyfrthseSrryImecitedHeresthegradateWereveryprdfysnAperfectreprtcardallsVeryprdMaIgtathingginghereYgtlintnyrfwThatsmeWavetsWelleinrwyearryItldystpflyinginthehseHeyAdamHeyarryIsthatfgelAlittleSpecialdaygradatinNeverthghtIdmakeitThreedaysgradeschlthreedayshighschlThsewereawkwardThreedayscllegeImgladItkadayandhitchhikedarndthehiveYdidcmeackdifferentHiarryArtiegrwingamstacheLksgdHearatFrankieYeahYgingtthefneralNImntgingEverydyknwsstingsmeneydieDntwasteitnasqirrelSchahtheadIgesshecldhavestgttentfthewayIlvethisincrpratinganamsementparkintrdayThatswhywedntneedvacatinsyqiteaitfpmpnderthecircmstancesWellAdamtdaywearemenWeareeemenAmenHallelahStdentsfacltydistingishedeespleasewelcmeDeanwellWelcmeNewHiveCitygradatingclassfThatcncldesrceremniesAndeginsyrcareeratHneIndstriesWillwepickrtdayIhearditsstrientatinHeadspHerewegKeepyrhandsandantennasinsidethetramatalltimesWnderwhatitllelikeAlittlescaryWelcmetHneadivisinfHnescandapartftheHeagnGrpThisisitWwWwWeknwthatyasaeehavewrkedyrwhlelifetgettthepintwhereycanwrkfryrwhlelifeHneyeginswhenrvaliantPllencksringthenectartthehivertpsecretfrmlaisatmaticallyclrcrrectedscentadstedandlecntredintthissthingsweetsyrpwithitsdistinctivegldenglwyknwasHneyThatgirlwashtShesmycsinSheisYeswereallcsinsRightYrerightAtHnewecnstantlystrivetimprveeveryaspectfeeeistenceTheseeesarestresstestinganewhelmettechnlgyWhatdythinkhemakesNtenghHerewehaverlatestadvancementtheKrelmanWhatdesthatdCatchesthatlittlestrandfhneythathangsafterypritSavessmillinsCananynewrkntheKrelmanfcrseMsteesaresmallnesteesknwthateverysmallifitsdnewellmeansalttchsecarefllyecaseyllstayintheypickfrtherestfyrlifeThesametherestfyrlifeIdidntknwthatWhatsthedifferenceYllehappytknwthateesasaspecieshaventhadnedayffinmillinyearsSyllstwrkstdeathWellsretryWwThatlewmymindWhatsthedifferenceHwcanysaythatnefreverThatsaninsanechicethavetmakeImrelievedNwwenlyhavetmakenedecisininlifetAdamhwcldtheyneverhavetldsthatWhywldyqestinanythingWereeesWerethemstperfectlyfnctiningscietynEarthYeverthinkmayethingswrkalittletwellhereLikewhatGivemeneeampleIdntknwtyknwwhatImtalkingatPleaseclearthegateRyalNectarFrcenapprachWaitasecndCheckittHeythsearePllencksWwIveneverseenthemthisclseTheyknwwhatitsliketsidethehiveYeahtsmedntcmeackHeycksHicksYgysdidgreatYremnstersYreskyfreaksIlveitIlveitIwnderwheretheywereIdntknwTheirdaysntplannedtsidethehiveflyingwhknwswheredingwhknwswhatYcantstdecideteaPllenckYhaveteredfrthatRightLkThatsmrepllenthanyandIwillseeinalifetimeItsstastatssymleesmaketmchfitPerhapsnlessyrewearingitandtheladiesseeywearingitThseladiesArenttheyrcsinstDistantDistantLkatthesetwCplefHiveHarrysLetshavefnwiththemItmstedangerseingaPllenckYeahnceaearpinnedmeagainstamshrmHehadapawnmythratandwiththetherhewasslappingmehmyIneverthghtIdknckhimtWhatwereydingdringthisTryingtalerttheathritiesIcanatgraphthatAlittlegstyttheretdaywasntitcmradesYeahGstyWerehittingasnflwerpatchsimilesfrmheretmrrwSimileshharryApddlempfrstmayeyrentpfritMayeIamYarentWeregingatGateWhatdythinkyyAreyeeenghImighteItalldependsnwhatmeansHeyHneDadysrprisedmeYdecidewhatyreinterestedinWelltheresaltfchicestynlygetneDyevergetreddingthesameeverydaySnletmetellyatstirringYgrathatstickandystmveitarndandystiritarndYgetyrselfintarhythmItsaeatiflthingYknwDadthemreIthinkatitmayethehneyfieldstisntrightfrmeYwerethinkingfwhatmakingallnanimalsThatsaadfragywithastingeranetyrsnsntsrehewantstginthneyarryyaresfnnysmetimesImnttryingtefnnyYrentfnnyYreginginthneyrsnthestirrerYregnnaeastirrerNneslisteningtmeWaittillyseethesticksIhaveIcldsayanythingrightnwImgnnagetananttattLetspensmehneyandcelerateMayeIllpiercemythraShavemyantennaeShackpwithagrasshpperGetagldtthandcalleverydydawgImsprdWerestartingwrktdayTdaysthedayCmenAllthegdswillegneYeahrightPllencntingstnteepringstirrerfrntdeskhairremvalIsitstillavailaleHa

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okay i did it! thank you again for your time and efforts

letter sequence in this ask matching protein-coding amino acids:

yeah no i'm not putting all that here again. i'm numbering the parts 1-8 in the above order

protein guy analysis:

parts 1 and 3-6 all look very similar. they're gigantic globular proteins, and with the exception of short loops between them, are composed entirely of alpha helices. easily the most notable thing about these structures is how massive they are – about 450 KDa each. i've gone ahead and put these into rainbow colours to help give some sense of depth. part 2 looks similar to the other blobs, but a lot less tightly compacted, with way more unstructured loops. it's the much more spaghetti-looking version of the others.

part 7 has different spaced out clusters that almost look like independent domains, although i may be biased by the colouring. part 8 is the most different, and is also a fair bit shorter than the others. its most distinct trait is the long sections of antiparallel beta sheets, which always remind me of amyloid fibrils. in case it wasn't clear, i absolutely hate seeing these things. i'd much rather have unstable spaghetti than super stable aggregates. there are three different sections of these beta sheet messes, forming either rectangular or triangular looking disasters.

the one consolation is that no significant part of any of these structures has a confidence score above 50%. or, in other words, even the backbone is uninterpretable and everything you're seeing here is just the mad hallucinations of a computer trying its best

predicted protein structure:

part 1

part 2

part 3

part 4

part 5

part 6

part 7

part 8

Hey

If you tell me what to do i could probably format the bee movie for it to be turned into a protein

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i'm going to regret this, but all you would need to do is remove all spaces, non-letter characters like punctuation and numbers, and all of the letters B, J, O, U, X and Z. *

* I also am not sure what the upper character limit is on tumblr asks or on AlphaFold 3 predictions, but if it was split into a few parts i'm sure it would be fine. if someone sends it to me in parts please also let me know so i can keep track of them all

letter sequence in this ask matching protein-coding amino acids:

HeyIfytellmewhattdicldpralyfrmattheeemviefrittetrnedintaprtein

protein guy analysis:

unlike the bee movie script, this is a short one. interestingly, it folded into a small beta sheet, which usually only appears on this blog within larger structures. however, when coloured by pLDDT score this whole structure turns yellow and orange. this is a nice aesthetic touch to match the bee movie, but it also shows that the confidence in this structure is incredibly low

predicted protein structure:

cartoon structure coloured by pLDDT

pLDDT colour code

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OP check your ask box

wow you were fast! ill try and get that post together soon, probably in the next day or two

hmm.. how about every heinz condiment/sauce?:

Mayonnaise

Light Mayonnaise

Garlic & Caramelized Onion Mayonnaise

Truffle Mayonnaise

Lemon & Black Pepper Mayonnaise

Salad Cream

Salad Cream 30% Less Fat

Salad Cream 70% Less Fat

Classic Barbecue Sauce

Sticky Barbecue Sauce

Sweet & Spicy Barbecue Sauce

American Style Smokey Baconnaise Sauce

Korean Style Sticky Barbecue Sauce

American Style Burger Sauce

Thai Style Sweet Chilli Sauce

Thai Style Coconut Lime Sauce

Turkish Style Garlic Sauce

Mild Yellow Mustard

Honey Yellow Mustard

Piccalilli Pickle

Ploughman's Pickle

Original Sandwich Spread

Light Sandwich Spread

Saucy Sauce (Mayo & Ketchup Sauce)

Heinz Tomato Ketchup

Aromatic Herbs Ketchup

Balsamic Vinegar Ketchup

Fiery Chilli Tomato Ketchup

Jalapeño Chilli Tomato Ketchup

Organic Tomato Ketchup

Roasted Garlic Ketchup

Sweet Chilli Tomato Ketchup

Firecracker

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growing up we had a family friend who worked for heinz, and he gave away free ketchup all the time. even then, i didn't know there were so many flavours of ketchup!

letter sequence in this ask matching protein-coding amino acids:

MaynnaiseLightMaynnaiseGarlicCarameliedninMaynnaiseTrffleMaynnaiseLemnlackPepperMaynnaiseSaladCreamSaladCreamLessFatSaladCreamLessFatClassicareceSaceStickyareceSaceSweetSpicyareceSaceAmericanStyleSmkeyacnnaiseSaceKreanStyleStickyareceSaceAmericanStylergerSaceThaiStyleSweetChilliSaceThaiStyleCcntLimeSaceTrkishStyleGarlicSaceMildYellwMstardHneyYellwMstardPiccalilliPicklePlghmansPickleriginalSandwichSpreadLightSandwichSpreadSacySaceMayKetchpSaceHeinTmatKetchpArmaticHersKetchpalsamicVinegarKetchpFieryChilliTmatKetchpalapenChilliTmatKetchprganicTmatKetchpRastedGarlicKetchpSweetChilliTmatKetchpFirecracker

protein guy analysis:

this reminds me of amyloid beta fibrils with the long beta sheet situation going on. maybe the repetition from the work 'ketchp' contributed to this? what's more interesting is that this makes up less than half of the structure. the rest of the protein is dominated by long alpha helices. the longest of these is found at the N terminus, and is 72 amino acids long! while some proteins do have longer helixes (eg. myosin), this is still well above the average alpha helix length of about 10 amino acids. overall, this is a very unique looking protein, and i wish it was real.

predicted protein structure:

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