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### =========================================================================
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### "sgedgesByGene" (and related) methods
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### -------------------------------------------------------------------------
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### - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
### intronsByTranscript()
###
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setMethod("intronsByTranscript", "SplicingGraphs", function(x) x@in_by_tx)
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### - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
### sgedgesByTranscript()
###
setGeneric("sgedgesByTranscript", signature="x",
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function(x, with.exon.mcols=FALSE, with.hits.mcols=FALSE)
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standardGeneric("sgedgesByTranscript")
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)
setMethod("sgedgesByTranscript", "SplicingGraphs",
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function(x, with.exon.mcols=FALSE, with.hits.mcols=FALSE)
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{
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if (!isTRUEorFALSE(with.exon.mcols))
stop("'with.exon.mcols' must be TRUE or FALSE")
if (!isTRUEorFALSE(with.hits.mcols))
stop("'with.hits.mcols' must be TRUE or FALSE")
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ex_by_tx <- unlist(x)
ex_partitioning <- PartitioningByEnd(ex_by_tx)
gene_ids <- names(ex_partitioning)
in_by_tx <- intronsByTranscript(x)
in_partitioning <- PartitioningByEnd(in_by_tx)
stopifnot(identical(gene_ids, names(in_partitioning)))
## Compute 'ans_partitioning'.
nex_by_tx <- width(ex_partitioning)
nin_by_tx <- width(in_partitioning)
stopifnot(identical(nin_by_tx + 1L, nex_by_tx))
ans_partitioning <- PartitioningByEnd(end(ex_partitioning) +
end(in_partitioning),
names=gene_ids)
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## Add missing metadata cols to 'in_unlistData'.
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ex_unlistData <- ex_by_tx@unlistData
ex_unlistData_len <- length(ex_unlistData)
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ex_unlistData_mcols <- mcols(ex_unlistData)
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check_exon_mcolnames(colnames(ex_unlistData_mcols))
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in_unlistData <- in_by_tx@unlistData
in_unlistData_len <- length(in_unlistData)
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in_unlistData_mcols <- mcols(in_unlistData)
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in_missing_mcols <- DataFrame(exon_id=NA_integer_,
exon_name=NA_character_,
exon_rank=NA_integer_,
start_SSid=NA_integer_,
end_SSid=NA_integer_)
idx <- rep.int(1L, in_unlistData_len)
in_missing_mcols <- in_missing_mcols[idx, , drop=FALSE]
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in_unlistData_mcols <- cbind(in_missing_mcols, in_unlistData_mcols)
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## Make "from" and "to" metadata cols.
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from <- as.character(ex_unlistData_mcols$start_SSid)
to <- as.character(ex_unlistData_mcols$end_SSid)
idx <- which(strand(ex_unlistData) == "-")
tmp <- from[idx]
from[idx] <- to[idx]
to[idx] <- tmp
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ex_prepend_mcols <- DataFrame(from=from, to=to)
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from <- to <- rep.int(NA_character_, in_unlistData_len)
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in_prepend_mcols <- DataFrame(from=from, to=to)
## Make "ex_or_in" and "tx_id" metadata cols.
ex_or_in <- rep.int(factor("ex", levels=EX_OR_IN_LEVELS),
ex_unlistData_len)
ex_prepend_mcols$ex_or_in <- ex_or_in
ex_or_in <- rep.int(factor("in", levels=EX_OR_IN_LEVELS),
in_unlistData_len)
in_prepend_mcols$ex_or_in <- ex_or_in
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tx_id <- mcols(ex_by_tx)[ , "tx_id"]
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ex_prepend_mcols$tx_id <- rep.int(tx_id, nex_by_tx)
in_prepend_mcols$tx_id <- rep.int(tx_id, nin_by_tx)
mcols(ex_unlistData) <- cbind(ex_prepend_mcols, ex_unlistData_mcols)
mcols(in_unlistData) <- cbind(in_prepend_mcols, in_unlistData_mcols)
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## Compute 'ans_unlistData'. We need to reorder 'c(ex_unlistData,
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## in_unlistData)' to bring introns between their flanking exons.
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ans_unlistData <- c(ex_unlistData, in_unlistData)
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seq0 <- seq_along(ex_partitioning)
roidx <- integer(ex_unlistData_len + in_unlistData_len)
seq1 <- seq_len(ex_unlistData_len)
idx1 <- seq1 * 2L - rep.int(seq0, nex_by_tx)
roidx[idx1] <- seq1
seq2 <- seq_len(in_unlistData_len)
idx2 <- seq2 * 2L + rep.int(seq0, nin_by_tx) - 1L
roidx[idx2] <- seq2 + ex_unlistData_len
ans_unlistData <- ans_unlistData[roidx]
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## Fill gaps in "from" and "to" metadata cols.
ans_unlistData_mcols <- mcols(ans_unlistData)
from <- ans_unlistData_mcols$from
to <- ans_unlistData_mcols$to
introns_idx <- which(is.na(from)) # same as 'which(is.na(to))'
from[introns_idx] <- to[introns_idx - 1L]
to[introns_idx] <- from[introns_idx + 1L]
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ans_unlistData_mcols$from <- from
ans_unlistData_mcols$to <- to
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## Sanity check: exons must be flanking introns.
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ans_unlistData_start <- start(ans_unlistData)
ans_unlistData_end <- end(ans_unlistData)
ans_unlistData_strand <- strand(ans_unlistData)
plus_idx <- which(ans_unlistData_strand == "+")
plus_introns_idx <- intersect(introns_idx, plus_idx)
stopifnot(identical(ans_unlistData_start[plus_introns_idx] - 1L,
ans_unlistData_end[plus_introns_idx - 1L]))
stopifnot(identical(ans_unlistData_end[plus_introns_idx] + 1L,
ans_unlistData_start[plus_introns_idx + 1L]))
minus_idx <- which(ans_unlistData_strand == "-")
minus_introns_idx <- intersect(introns_idx, minus_idx)
stopifnot(identical(ans_unlistData_start[minus_introns_idx] - 1L,
ans_unlistData_end[minus_introns_idx + 1L]))
stopifnot(identical(ans_unlistData_end[minus_introns_idx] + 1L,
ans_unlistData_start[minus_introns_idx - 1L]))
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## Insert "sgedge_id" metadata col after first 2 metadata cols
## ("from" and "to").
sgedge_id <- make_global_sgedge_id(
rep.int(gene_ids, width(ans_partitioning)),
from, to)
ans_unlistData_mcols <- c(ans_unlistData_mcols[1:2],
DataFrame(sgedge_id=sgedge_id),
ans_unlistData_mcols[-(1:2)])
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check_all_edge_mcolnames(colnames(ans_unlistData_mcols))
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## Drop unwanted columns.
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mcol_idx <- get_index_of_group_of_mcols(
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colnames(ans_unlistData_mcols),
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with.exon.mcols, with.hits.mcols)
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if (length(mcol_idx) != 0L)
ans_unlistData_mcols <- ans_unlistData_mcols[ , -mcol_idx,
drop=FALSE]
mcols(ans_unlistData) <- ans_unlistData_mcols
## Relist 'ans_unlistData' and return.
ans <- relist(ans_unlistData, ans_partitioning)
ans
}
)
### - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
### sgedgesByGene()
###
setGeneric("sgedgesByGene", signature="x",
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function(x, with.exon.mcols=FALSE, with.hits.mcols=FALSE,
keep.dup.edges=FALSE)
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standardGeneric("sgedgesByGene")
)
setMethod("sgedgesByGene", "SplicingGraphs",
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function(x, with.exon.mcols=FALSE, with.hits.mcols=FALSE,
keep.dup.edges=FALSE)
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{
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if (!isTRUEorFALSE(keep.dup.edges))
stop("'keep.dup.edges' must be TRUE or FALSE")
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edges_by_tx <- sgedgesByTranscript(x, with.exon.mcols=with.exon.mcols,
with.hits.mcols=with.hits.mcols)
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edges0 <- unlist(edges_by_tx)
edges0_mcols <- mcols(edges0)
edges0_mcolnames <- colnames(edges0_mcols)
sgedge_id <- edges0_mcols[ , "sgedge_id"]
sm <- match(sgedge_id, sgedge_id)
## Sanity checks.
stopifnot(all(edges0 == edges0[sm]))
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invar_mcol_idx <- get_index_of_invariant_edge_mcols(edges0_mcolnames)
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stopifnot(identical(
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edges0_mcols[ , invar_mcol_idx, drop=FALSE],
edges0_mcols[sm , invar_mcol_idx, drop=FALSE]))
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## Compute 'ans_partitioning'.
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ans_unlistData <- edges0
if (!keep.dup.edges) {
keep_idx <- which(sm == seq_along(sm))
ans_unlistData <- ans_unlistData[keep_idx]
}
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ans_grouping <- Rle(names(ans_unlistData))
ans_eltlens <- runLength(ans_grouping)
ans_partitioning <- PartitioningByEnd(cumsum(ans_eltlens),
names=runValue(ans_grouping))
## Compute 'ans_unlistData'.
names(ans_unlistData) <- NULL
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if (!keep.dup.edges) {
ans_unlistData_mcols <- mcols(ans_unlistData)
var_mcol_idx <- seq_along(edges0_mcolnames)[- invar_mcol_idx]
f <- factor(sgedge_id, levels=sgedge_id[keep_idx])
for (i in var_mcol_idx) {
old_col <- edges0_mcols[ , i]
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new_col <- unname(unique(unlistAndSplit(old_col, f)))
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ans_unlistData_mcols[ , i] <- new_col
}
mcols(ans_unlistData) <- ans_unlistData_mcols
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}
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## Relist 'ans_unlistData' and return.
ans <- relist(ans_unlistData, ans_partitioning)
ans
}
)
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