Search Results for "microscopy"
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Showing 80 open source projects for "microscopy"
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AtomAI
Deep and Machine Learning for Microscopy
AtomAI is a Pytorch-based package for deep and machine-learning analysis of microscopy data that doesn't require any advanced knowledge of Python or machine learning. The intended audience is domain scientists with a basic understanding of how to use NumPy and Matplotlib. It was developed by Maxim Ziatdinov at Oak Ridge National Lab. The purpose of the AtomAI is to provide an environment that bridges the instrument-specific libraries and general physical analysis by enabling the seamless deployment of machine learning algorithms including deep convolutional neural networks, invariant variational autoencoders, and decomposition/unmixing techniques for image and hyperspectral data analysis. ... -
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Gwyddion
Scanning probe microscopy data visualisation and analysis
A data visualization and processing tool for scanning probe microscopy (SPM, i.e. AFM, STM, MFM, SNOM/NSOM, ...) and profilometry data, useful also for general image and 2D data analysis. -
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libgwyscan
Scanning probe microscopy support library
Gwyscan is scanning probe microscopy support library, including support for equidistant and non-equidistant scanning path creation, non-equidistant data pre-processing and saving different files obtained during scanning in Gwyddion compatible format. The work was supported by an EMRP project "6 Degrees of Freedom" jointly funded by the EMRP participating countries within EURAMET and the European Union. -
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computem
Transmission Electron Microscope Image Simulation
These programs calculate high resolution conventional and scanning transmission electron microscope (CTEM, STEM) images of thin specimens from first principles using the multislice method for electrons in the energy range of approximately 60 keV to 1000 keV. The fundamental theory and usage are described in "Advanced Computing in Electron Microscopy" (Springer 2020) and Acta Cryst. A72 (2016) p. 1 by Earl J. Kirkland. Please refer to these for the theory of calculation and how to use these programs. computem uses a GUI and the temsim group uses a command line interface. The user is assumed to have some understanding of optics, Fourier transforms, electron microscopy and computer skills at the graduate or advanced undergraduate level. ... -
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CellMAP
CellMAP: an open-source software tool to batch-process cells from AFM
CellMAP: an open-source software tool to batch-process cell topography and elasticity maps collected by atomic force microscopy. -
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CometAnalyser
CometAnalyser, for quantitative comet assay analysis.
Description: Comet assay provides an easy solution to estimate DNA damage in single cells through microscopy assessment. To obtain reproducible and reliable quantitative data, we developed an easy-to-use tool named CometAnalyser. CometAnalyser is an open-source deep-learning tool designed for the analysis of both fluorescent and silver-stained wide-field microscopy images. Once the comets are segmented and classified, several intensity/morphological features are automatically exported as a spreadsheet file. ... -
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ReViMS
ReViMS, a 3D volume rendering tool for light-sheet/confocal microscopy
Reconstruction and Visualization from Multiple Sections (ReViMS), an open-source, user-friendly software for automatically estimating volume and several other features of 3D multicellular aggregates (i.e., cancer spheroid, zebrafish, fruit fly). ReViMS requires a z-stack of 2D binary masks, obtained by segmenting a sequence of fluorescent images acquired by scanning the aggregate along the z axis, using a confocal or a light-sheet fluorescent microscope. It provides a number of tools... -
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Gwyscope
Open hardware SPM controller with advanced sampling support.
Gwyscope is a low cost, open hardware, Digital Signal Processor (DSP) suitable for Scanning Probe Microscopy measurements, focusing on demonstrating the concept of adaptive scanning, general XYZ data acquisition and statistical data processing on the controller level. More details can be found in: M. Valtr et al., Scanning Probe Microscopy controller with advanced sampling support, HardwareX, Volume 15, e00451 https://www.hardware-x.com/article/S2468-0672(23)00058-5/fulltext It is based on a low cost FPGA board Red Pitaya and additional high bit depth AD and DA converters. ... -
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TomoJ
ImageJ plugin to perform Electron Tomography
...reconstruction part was described in: BMC Bioinformatics. 2007 Aug 6;8:288. "TomoJ: tomography software for three-dimensional reconstruction in transmission electron microscopy."Messaoudi C et al -
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Thumbnailer for SXM files
Shell Extension for SXM files for Nanonis SPM images
If you do scanning probe microscopy using Nanonis controllers then this application may come in handy. This is a shell extension for Windows (Vista, 7 , 8 and above) to enable visualisation of your SXM image files in windows explorer. Hovering over the file also shows a 'tooltip' with information about the image scan size and feedback parameters used. Please make sure you download the correct version, 32 or 64 bit, depending on your computer OS version. -
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ColorI-DT
A tool for the quantitative evaluation of differences in color images
...Contacts: Filippo Piccinini (emails: filippo.piccinini@irst.emr.it; f.piccinini@unibo.it) Repository: https://sourceforge.net/p/colori-dt/ How to cite us when using the tool: Filippo Piccinini et al. “Colori-DT: an open-source tool for the quantitative evaluation of differences in microscopy color images”. -
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Image Tensors
Convert Lif, Czi, Metamorph ND, Oib to TIFF with ImageJ compatibility.
Image Tensors automates the conversion of various microscopy image formats (Lif, Czi, Metamorph ND, Oib) into a uniform TIFF format. Part of the larger BioPixel project, this utility is released for public use while BioPixel is under development. It benefits researchers and scientists requiring efficient image format conversion. Key Features: Support for Multiple Formats: Converts Lif, Czi, Metamorph ND, and Oib. -
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GSvit
Fast FDTD solver with graphics card support
Fast FDTD solver with graphics card support. Optimized for nanoscale optics - scanning near field optical microscopy, rough surface scattering and solar cells. Uses CUDA environment for graphics card operation. -
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ACORBA
Automated approach to measure root tip angles of Arabidopsis thaliana
Gravitropic response is studied in most of the laboratories working with Arabidopsis thaliana, for example, to detect new phenotypes in mutants. However, manual analysis of images and microscopy data are known to be subjected to human bias. This is particularly the case for manual measurements of root bending as the angle is set subjectively. In this context, it is essential to develop and use automated or semi-automated image analysis to produce faster, reproducible, and unbiased data. In this context, we developped ACORBA (Automatic Calculation Of Root Bending Angles), a fully automated software to measure root bending angle over time. -
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GXSM
Scanning Probe Microscopy Controller and Data Visualization Software
GXSM -- Gnome X Scanning Microscopy: A multi-channel image and vector-probe data acquisition and visualization system designed for SPM techniques (STM,AFM..), but also SPA-LEED/LEED/LEEM data analysis. A plug-in interface allows any user add-on data-processing and special hardware and instrument support. Latest: NC-AFM and related explorative methods as SQDM can be configured. -
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AtomicJ
Application for analysis of AFM data
AtomicJ is an application for analysis of force microscopy recordings, including images and force curves. It allows for fast and reliable processing of single force curves and force maps, providing estimation of the mechanical properties of the sample. AtomicJ supports a wide range of contact mechanics models, including the models for blunt tips, the corrections for the effect of finite sample thickness, models of adhesive contact and models of spherical indentation of hyperelastic material. ... -
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coDDMaker
distributions and co-distribution 2D signal analysis
coDDMaker is a MATLAB® App Designer's software program for the guided analysis of the distributions and co-distribution of marker pairs in 2D image. -
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DDMaker
Local Distribution Density Map Maker
DDMaker perform local density analysis and generates pseudocolor maps of the spatial distribution of imaged cellular structures in 2D images, starting from either RGB color, grey level or binary images. DDMaker local density analysis permit to selectively denoise the signal, visualize and quantify its distribution and threshold the image basing on local density. -
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ANDTool
Analysis Nuclei DAB (AND) Tool
Analysis Nuclei DAB (AND) Tool is a Graphical User Interface (GUI) to analyse microscopy images representing cells with nuclei stained using DAB dyes. The tool requires as input the original RGB images, and the FastRed, FastBlue, DAB channel, easily obtained using the Fiji function: "ImageJ" -> "Image" -> "Colour Deconvolution" -> "FastRed FastBlue DAB" Then, the tool first segment the nuclei using the FastBlue channel and the DAB channel, and then computes statistics by subdividing the sample in three regions according to the FastRed channel: a dark-red ROI, a light-pink ROI and a white ROI. ... -
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DSeg software
A MATLAB program to segment filamentous bacteria and hyphae structures
The analysis of microscopy image has been the basis to our current understanding of the cellular growth and morphogenesis. The quantitative evaluation of morphological changes in the biological processes is therefore important to characterize cell structures. Here we present an image analysis tool DSeg to overcome the difficulties in finding complicated elongated cell shapes by using time-lapse data and cell morphological constraints. -
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MORPHEUS for Fiji
A tool for unbiased and reproducible cell morphometry in Fiji/ImageJ2
MORPHEUS is a Fiji/ImageJ2 plugin for the automated evaluation of cell morphometry from cell cultures images acquired by fluorescence microscopy. Specifically, MORPHEUS works with sampling distributions to learn—in an unsupervised manner and by a non-parametric approach—how to recognize the cells suitable for subsequent analysis. Afterwards, the algorithm performs the evaluation of the most relevant cell-shape descriptors over the full set of detected cells. Optionally, also the extraction of nucleus features and a double-scale analysis of orientation can be performed. ... -
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VividSTORM
Correlated confocal and SMLM data visualization and analysis
VividSTORM is a free and open-source standalone software with graphical user interface, for the correlated visualization and analysis of superresolution single molecule localization microscopy (SMLM) molecule lists and conventional pixel intensity-based images. The localization points (LPs) within this ROI can be analyzed using the selected built-in functions. NOTE: If you encounter issues not addressed by the user guide, please contact by message on this site or via e-mail for additional support: vividstorm[at]koki.mta.hu If you find our software useful in your project, please cite: Correlated confocal and super-resolution imaging by VividSTORM Nature Protocols 11, 163–183 (2016) doi:10.1038/nprot.2016.002 -
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...We are constantly updating the code to implement other file formats as well - suggestions are welcome. Main articles: -) TXM-Wizard: a program for advanced data collection and evaluation in full-field transmission X-ray microscopy, Journal of Synchrotron Radiation, 19, 281-287. (2012) http://scripts.iucr.org/cgi-bin/paper_yard?hf5192 -) 3D elemental sensitive imaging using transmission X-ray microscopy, Analytical and Bioanalytical Chemistry, Volume 404, Issue 5, pp 1297-1301 (2012) http://link.springer.com/article/10.1007%2Fs00216-012-5818-9 -) Three-dimensional imaging of chemical phase transformations at the nanoscale with full-field transmission X-ray microscopy, Journal of Synchrotron Radiation, 18, 773-781. (2011) http://scripts.iucr.org/cgi-bin/paper?...
